pe conjugated anti cd3 Search Results


anti human cd3  (Diaclone)
92
Diaclone anti human cd3
Anti Human Cd3, supplied by Diaclone, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/Anti-Human+CD3+Monoclonal+Antibody%2C+PE+Conjugated+Clone+B-B11/pmc03209779-440-0-9
Average 92 stars, based on 1 article reviews
anti human cd3 - by Bioz Stars, 2026-11
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fluorochrome-conjugated antibodies anti-cd3/fitc im2635  (Immunotec inc)
90
Immunotec inc fluorochrome-conjugated antibodies anti-cd3/fitc im2635
Fluorochrome Conjugated Antibodies Anti Cd3/Fitc Im2635, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/pe+cy5+conjugated+mouse+anti+human+cd3+mab+im2635u/pmc04177855-60-12-14
Average 90 stars, based on 1 article reviews
fluorochrome-conjugated antibodies anti-cd3/fitc im2635 - by Bioz Stars, 2026-11
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pe-conjugated anti-cluster of differentiation 3 (cd3) mab  (Becton Dickinson)
90
Becton Dickinson pe-conjugated anti-cluster of differentiation 3 (cd3) mab
Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated <t>anti-CD3</t> mAb and analyzed for expression of Vδ2 and <t>CD3</t> through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.
Pe Conjugated Anti Cluster Of Differentiation 3 (Cd3) Mab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/pe+conjugated+anti+cluster+of+differentiation+3++cd3++mab/pmc06818088-197-27-62
Average 90 stars, based on 1 article reviews
pe-conjugated anti-cluster of differentiation 3 (cd3) mab - by Bioz Stars, 2026-11
90/100 stars
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monoclonal (pe)-conjugated anti-mouse cd3  (Becton Dickinson)
90
Becton Dickinson monoclonal (pe)-conjugated anti-mouse cd3
Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated <t>anti-CD3</t> mAb and analyzed for expression of Vδ2 and <t>CD3</t> through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.
Monoclonal (Pe) Conjugated Anti Mouse Cd3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/monoclonal++pe++conjugated+anti+mouse+cd3/pm11886253-128-2-10
Average 90 stars, based on 1 article reviews
monoclonal (pe)-conjugated anti-mouse cd3 - by Bioz Stars, 2026-11
90/100 stars
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anti-human cd3-pe conjugated antibodies  (Sony)
90
Sony anti-human cd3-pe conjugated antibodies
Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated <t>anti-CD3</t> mAb and analyzed for expression of Vδ2 and <t>CD3</t> through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.
Anti Human Cd3 Pe Conjugated Antibodies, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/anti+human+cd3+pe+conjugated+antibodies/pmc11049294-41-8-12
Average 90 stars, based on 1 article reviews
anti-human cd3-pe conjugated antibodies - by Bioz Stars, 2026-11
90/100 stars
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anti-human cd3 clone sk7 conjugated with phycoerythrin (pe)  (Becton Dickinson)
90
Becton Dickinson anti-human cd3 clone sk7 conjugated with phycoerythrin (pe)
Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated <t>anti-CD3</t> mAb and analyzed for expression of Vδ2 and <t>CD3</t> through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.
Anti Human Cd3 Clone Sk7 Conjugated With Phycoerythrin (Pe), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/anti+human+cd3+clone+sk7+conjugated+with+phycoerythrin++pe+/pm16099011-148-54-14
Average 90 stars, based on 1 article reviews
anti-human cd3 clone sk7 conjugated with phycoerythrin (pe) - by Bioz Stars, 2026-11
90/100 stars
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pe-conjugated anti-cd3 145-2c11  (Millennium Pharmaceuticals)
90
Millennium Pharmaceuticals pe-conjugated anti-cd3 145-2c11
Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated <t>anti-CD3</t> mAb and analyzed for expression of Vδ2 and <t>CD3</t> through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.
Pe Conjugated Anti Cd3 145 2c11, supplied by Millennium Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/pe+conjugated+anti+cd3+145+2c11/pmc02194171-64-11-21
Average 90 stars, based on 1 article reviews
pe-conjugated anti-cd3 145-2c11 - by Bioz Stars, 2026-11
90/100 stars
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pe-conjugated anti-cd3  (MBL Life science)
90
MBL Life science pe-conjugated anti-cd3
Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated <t>anti-CD3</t> mAb and analyzed for expression of Vδ2 and <t>CD3</t> through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.
Pe Conjugated Anti Cd3, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pe+conjugated+anti+cd3/pe+conjugated+anti+cd3/pm33886156-79-18-32
Average 90 stars, based on 1 article reviews
pe-conjugated anti-cd3 - by Bioz Stars, 2026-11
90/100 stars
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mouse anti cd3, conjugated to pe  (Nordic-Mubio)
N/A
The CD3 mAb (clone UCHT1) recognizes cytoplasmic CD3 epsilon in precursor T-cells and cytoplasmic and surface CD3 epsilon in mature T-lymphocytes. The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative
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anti-human cd3 cd3e monoclonal antibody pe-cy7 conjugated, flow validated  (Boster Bio)
N/A
Boster Bio Anti-Human CD3 CD3E Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated (Catalog# FC02675-PE-Cy7). Tested in Flow Cytometry application(s). This antibody reacts with Human.
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anti human cd3 cd3d monoclonal antibody pe conjugated flow validated  (Boster Bio)
N/A
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anti-human cd3 monoclonal antibody pe conjugated, flow validated  (Boster Bio)
N/A
Boster Bio Anti-Human CD3 Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC02675-PE-1). Tested in Flow Cytometry application(s). This antibody reacts with Human.
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Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated anti-CD3 mAb and analyzed for expression of Vδ2 and CD3 through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.

Journal: ChemMedChem

Article Title: Synthesis and immunomodulatory activity of fluorine-containing bisphosphonates

doi: 10.1002/cmdc.201800764

Figure Lengend Snippet: Immunomodulatory effects of tetrakispivaloxylmethyl-1-fluoro-2-(1H-imidazol-1-yl)ethylidene-1,1-bisphosphonate (11) on Vγ2Vδ2 T cells derived from a lung cancer patient. (A) Expansion by 11 of Vγ2Vδ2 T cells from PBMCs derived from a patient with lung cancer. PBMCs were stimulated with 11 and cell clustering was assessed on days 4, 5, 6, and 7. (B) Flow cytometric analysis of Vγ2Vδ2 T cells before (day 0) and after (day 11) expansion with 11. Cells on days 0 and 11 were stained with FITC-conjugated anti-Vδ2 mAb and PE-conjugated anti-CD3 mAb and analyzed for expression of Vδ2 and CD3 through a FACS Verse flow cytometer. (C) Specific lysis of PC-9 lung carcinoma cells by compound 11-expanded Vγ2Vδ2 T cells. (Left panel) Spontaneous and maximal time-resolved fluorescence of PC-9 cells. PC-9 cells were treated with 25 μM of BM-HT at 37oC for 15 min. After washing, the cells were incubated in the absence (spontaneous) or presence (maximum) of 0.125% detergent in DMSO for 40 min. Supernatants from the cultures were then mixed with Eu3+ solution and the spontaneous and maximum release determined by measuring time-resolved fluorescence using a PHERAStar multiplate spectrophotometer. The spontaneous release rate was 18% (Right panel) Specific lysis of PC-9 cells by Vγ2Vδ2 T cells. PC-9 cells were pretreated with 0 μM (♦), 1.25 μM (■), 2.5 μM (▲), or 5 μM (●) of 11 at 37oC for 2 h. The cells were further treated with 25 μM of BM-HT at 37oC for 15 min. The specific lysis of pretreated-PC-9 cells by Vγ2Vδ2 T cells expanded by compound 11 was determined as in Fig. 2. Data show mean ± SD and are representative of three independent experiments.

Article Snippet: The cells were incubated with 3 μL of fluorescein isothiocyanate (FITC)-conjugated TCR Vδ2 mAb (BD Biosciences, San Diego, CA) and 3 μL of phycoerythrin (PE)-conjugated anti-cluster of differentiation 3 (CD3) mAb on ice for 15 min. After being washed three times with 200 μL of PBS, the cells were resuspended in 600 μL of PBS and analyzed using a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ).

Techniques: Derivative Assay, Staining, Expressing, Flow Cytometry, Lysis, Fluorescence, Incubation, Spectrophotometry